Journal: Redox Biology

Article Title: Combination of YAP inhibition and photodynamic therapy induces dual DNA damage and activates STING pathway to enhance immunotherapy in uveal melanoma

doi: 10.1016/j.redox.2025.103965

Figure Lengend Snippet: Activation of the cGAS-STING pathway by HANP/VP via YAP-inhibition and PDT in vitro. (A) The phosphorylation levels of representative proteins of the cGAS-STING pathway were analyzed using Western blot ( left ) and quantified ( right ). After HANP/VP treatment without laser irradiation, the pSTING/STING, pTBK1/TBK1, and pIRF3/IRF3 ratio were significantly increased compared to the untreated cells; the phosphorylation levels were further increased after laser irradiation, n = 3/group. (B) qPCR was used to detect IFN-β1 mRNA levels. Compared with the control group, HANP/VP without Laser treatment significantly increased IFN-β1 expression in B16F10 cells, while HANP/VP combined with laser irradiation further elevated IFN-β1 mRNA levels. When cells were pretreated with ethidium bromide (EB, 120 ng/mL), IFN-β1 mRNA levels decreased in the HANP/VP with Laser irradiation group. Following H151 treatment, IFN-β1 mRNA levels decreased to control group levels regardless of laser irradiation status. n = 3/group. Statistical significance was determined by one-way ANOVA (∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: The mouse melanoma cell line B16F10 was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Activation Assay, Inhibition, In Vitro, Phospho-proteomics, Western Blot, Irradiation, Control, Expressing

Journal: Glycobiology

Article Title: Editor's Choice Functional inactivation of oligosaccharyltransferase a isoform suppresses tumor metastasis

doi: 10.1093/glycob/cwag003

Figure Lengend Snippet: Syngeneic model. (A) Schematic representation of the experimental model. When cancer cell-derived EVs that contain Cre25nt mRNA were incorporated into recipient cells, the mRNA may be translated to Cre recombinase, leading to expression of tdTomato protein. (B and C) RT-PCR and western blot analysis of Cre25nt mRNA (B) and Cre protein (C) in wild-type (WT) B16-F10 cells, Cre25nt cells, and small EVs secreted from these cells. (D) Expression of tdTomato (red) in tumors formed by WT cells and Cre25nt cells. tdTomato protein was also detected by anti-red fluorescent protein (RFP) antibody (green). Sections were counterstained with Hoechst 33342 (cyan). Scale bars, 100 μm.

Article Snippet: The mouse melanoma cell line B16-F10 was purchased from American Type Culture Collection (ATCC).

Techniques: Derivative Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Glycobiology

Article Title: Editor's Choice Functional inactivation of oligosaccharyltransferase a isoform suppresses tumor metastasis

doi: 10.1093/glycob/cwag003

Figure Lengend Snippet: Gene-edited knockout of the Stt3a and Stt3b genes in mouse melanoma cells. (A and B) Western blot analysis of wild-type (WT) B16-F10, Stt3a -RE, Stt3a -KO, Stt3b -RE, and Stt3b -KO cells. Samples were treated with or without PNGase F (B). (C) Quantitative real-time PCR analysis of Cre25nt mRNA in small EVs secreted from Stt3a -RE, Stt3a -KO, Stt3b -RE, and Stt3b -KO cells. Average expression of Stt3a -KO, Stt3b -KO were set to 1.0. Data represent the mean ± standard deviation; n = 3.

Article Snippet: The mouse melanoma cell line B16-F10 was purchased from American Type Culture Collection (ATCC).

Techniques: Knock-Out, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation

Journal: bioRxiv

Article Title: Volumetric mechanoplasticity couples melanoma drug tolerance to susceptibility to CD8⁺ T cell killing

doi: 10.64898/2026.01.27.701903

Figure Lengend Snippet: (A) Representative nuclear staining images of primed B16F10 cells stained with DAPI across ISO and primed 50DI. Scale bar as indicated. (B) Single nucleus quantification of normalized DAPI intensity in primed B16F10 nuclei (n ≥ 20 per condition from 4 independent experiments). (C) GSEA for a chromosome condensation gene set comparing adapted 25DI versus ISO and adapted 50DI versus ISO in B16F0. (D) Heatmap of selected stress and p53 related target genes across ISO, adapted 25DI, and adapted 50DI from B16F0 RNA sequencing. Values are displayed as z scored expression across samples. Scatter plots show mean ± s.d. All RNA sequencing analyses were performed on adapted cells (5 day exposure) and compared to ISO.

Article Snippet: B16F0 and B16F10 mouse melanoma cells were obtained from ATCC.

Techniques: Staining, RNA Sequencing, Expressing

Journal: bioRxiv

Article Title: Volumetric mechanoplasticity couples melanoma drug tolerance to susceptibility to CD8⁺ T cell killing

doi: 10.64898/2026.01.27.701903

Figure Lengend Snippet: (A) Brightfield images showing B16F10 morphology during adaptation in ISO, 25DI, and 50DI at day 1, day 3, and day 5. (B) Cell number fold change over five days in ISO, adapted 25DI, and adapted 50DI. (C) Day 5 over day 0 fold change (log10) in each condition (n = 8). (D) Heatmap of representative G1S, G2M, and M phase regulators across ISO, adapted 25DI, and adapted 50DI from RNA sequencing. Heatmap color bars show z scored expression. (E) Post recovery confocal images of ISO and primed 50DI B16F10 monolayers during isotonic regrowth, with cancer cell masks shown in cyan. (F) Time course of percent area covered by cancer cells over 36 h in ISO and primed 50DI. (G) Cancer cell area at 36 h in ISO and primed 50DI (n ≥ 20 images per condition from 4 independent experiments). Scatter plots show mean ± s.d. All RNA sequencing analyses were performed on adapted cells (5 day exposure) and compared to ISO.

Article Snippet: B16F0 and B16F10 mouse melanoma cells were obtained from ATCC.

Techniques: RNA Sequencing, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: ERM proteins support perinuclear actin rim formation

doi: 10.3389/fcell.2025.1579946

Figure Lengend Snippet: Perinuclear localization of ERM proteins. Confluent B16–F10 cells induced to migrate in the wound healing assay for 3 h, immunostained for Radixin (A) , Moesin (B) , and Ezrin (C) , along with Lamin B. DNA is stained with Hoechst. The edge of the scratch is in the top region of each micrograph. The nuclei in the orange rectangles are magnified. Scale bar: 20 µm. For quantification, 20–45 cells from each condition were measured in each experiment for the ERM protein signal at the nuclear periphery. The mean intensity was calculated and normalized to control cells. The average mean intensity in three independent experiments ±s.e. is presented. Statistical significance was evaluated by the Student’s t-test, * P < 0.05.

Article Snippet: Mouse melanoma B16-F10 cell line purchased from ATCC was grown as described previously ( ).

Techniques: Wound Healing Assay, Staining, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: ERM proteins support perinuclear actin rim formation

doi: 10.3389/fcell.2025.1579946

Figure Lengend Snippet: Overexpression of ERM proteins increases the intensity of the perinuclear actin rim. (A) Perinuclear actin rim upon overexpression of ERM proteins. Confluent B16-F10 cells over-expressing GFP-fused ERM proteins were induced to migrate in the wound healing assay for 3 h, stained for filamentous actin (Phalloidin), nuclear envelope (Lamin B), and DNA (Hoechst). The edge of the scratch is in the top region of each micrograph. The nuclei in the orange rectangles are magnified on the left side. Scale bar: 20 µm. (B) Quantification of the actin perinuclear rim in ERM overexpressing cells vs. control cells. For quantification, 20–30 cells from each condition were measured for the Phalloidin signal at the nuclear periphery in each experiment. The mean intensity was calculated and normalized to control cells. The average mean intensity in three independent experiments ±s.e. is presented. Statistical significance was evaluated by the Student’s t-test, * P < 0.05, ** P < 0.01.

Article Snippet: Mouse melanoma B16-F10 cell line purchased from ATCC was grown as described previously ( ).

Techniques: Over Expression, Expressing, Wound Healing Assay, Staining, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: ERM proteins support perinuclear actin rim formation

doi: 10.3389/fcell.2025.1579946

Figure Lengend Snippet: ERM proteins support the formation of a perinuclear actin rim. (A) Actin perinuclear rim after KD of ERM proteins. Sub-confluent B16–F10 cells transfected with either control, Radixin, Moesin, or Ezrin siRNA stained for filamentous actin (Phalloidin), nuclear envelope (Lamin B), and DNA (Hoechst). The nuclei in the orange rectangles are magnified on the left side. Scale bar: 20 µm. (B) Quantification of the actin perinuclear rim in siRNA ERM proteins vs. siRNA Control transfected B16–F10 cells. For quantification, in each experiment, 20–30 cells of each transfection were measured for the Phalloidin signal at the nuclear periphery. The mean intensity was calculated and normalized to control cells. The average mean intensity in three independent experiments ±s.e. is presented. Statistical significance was evaluated by the Student’s t-test, ** P < 0.01.

Article Snippet: Mouse melanoma B16-F10 cell line purchased from ATCC was grown as described previously ( ).

Techniques: Transfection, Control, Staining